factor κb ligand rankl  (Novoprotein)

Journal: International Journal of Molecular Medicine

Article Title: TWEAK modulates the characteristics of periodontal ligament stem cells via the Fn14/NF-κB pathway

doi: 10.3892/ijmm.2025.5679

Figure Lengend Snippet: Effects of tumor necrosis factor-like weak inducer of apoptosis on RANKL and OPG expression in PDLSCs and PDLSC-induced macrophage polarization. (A) RANKL (red) and OPG (green) expression in PDLSCs was detected by immunofluorescence staining. Scale bar, 200 μ m. Statistical analysis of the MOD values for (B) RANKL and (C) OPG, and (D) their ratio based on (A) (E) CD68 and CD163 expression in RAW264.7 macrophages was assessed by immunofluorescence staining. Scale bar, 200 μ m. Statistical analysis of the MOD values for (F) CD68 and (G) CD163 based on (E). Statistical analysis was performed using a one-way ANOVA. *** P<0.001; **** P<0.0001. Data are presented as the mean ± SD (n=5). MOD, mean optical density; ns, not significant; OPG, osteoprotegerin; PDLSC, periodontal ligament stem cell; RANKL, receptor activator of nuclear factor-κB ligand.

Article Snippet: The primary antibodies used for immunofluorescence staining included the mouse anti-receptor activator of nuclear factor-κB ligand (RANKL) antibody (cat. no. sc-52950; Santa Cruz Biotechnology, Inc.), rabbit anti-osteoprotegerin (OPG) antibody (cat. no. ab73400; Abcam), mouse anti-CD68 antibody (cat. no. ab201340; Abcam) and rabbit anti-CD163 antibody (cat. no. ab182422; Abcam).

Techniques: Expressing, Immunofluorescence, Staining

Journal: International Journal of Molecular Medicine

Article Title: TWEAK modulates the characteristics of periodontal ligament stem cells via the Fn14/NF-κB pathway

doi: 10.3892/ijmm.2025.5679

Figure Lengend Snippet: Inhibition of Fn14 and NF-κB effectively blocks TWEAK-induced alterations in the microenvironmental regulatory potential of PDLSCs. (A) Expression levels of OPG (green) and RANKL (red) in PDLSCs were detected by immunofluorescence staining, followed by quantitative analysis of the MOD values for (B) RANKL and (C) OPG, and (D) the MOD ratio of RANKL/OPG (n=5). Scale bar, 200 μ m. (E) Expression levels of CD68 and CD163 in RAW264.7 macrophages were detected by immunofluorescence staining, followed by quantitative analysis of the MOD values for (F) CD68 and (G) CD163 (n=5). Scale bar, 200 μ m. Statistical analysis was performed using a one-way ANOVA. ** P<0.01; *** P<0.001; **** P<0.0001. Data are presented as the mean ± SD. Fn14, fibroblast growth factor-inducible 14; MOD, mean optical density; ns, not significant; OPGa, osteoprotegerin; PDLSC, periodontal ligament stem cell; RANKL, receptor activator of nuclear factor-κB ligand; sh, short hairpin RNA; TWEAK, tumor necrosis factor-like weak inducer of apoptosis.

Article Snippet: The primary antibodies used for immunofluorescence staining included the mouse anti-receptor activator of nuclear factor-κB ligand (RANKL) antibody (cat. no. sc-52950; Santa Cruz Biotechnology, Inc.), rabbit anti-osteoprotegerin (OPG) antibody (cat. no. ab73400; Abcam), mouse anti-CD68 antibody (cat. no. ab201340; Abcam) and rabbit anti-CD163 antibody (cat. no. ab182422; Abcam).

Techniques: Inhibition, Expressing, Immunofluorescence, Staining, shRNA

Journal: International Journal of Molecular Medicine

Article Title: TWEAK modulates the characteristics of periodontal ligament stem cells via the Fn14/NF-κB pathway

doi: 10.3892/ijmm.2025.5679

Figure Lengend Snippet: Effect of inhibition of the tumor necrosis factor-like weak inducer of apoptosis/Fn14/NF-κB/NLRP3 pathway on the microenvironmental regulatory ability of iPDLSCs. (A) Immunofluorescence staining was used to detect the expression levels of RANKL and OPG in PDLSCs, iPDLSCs, and iPDLSCs after the downregulation of Fn14, NF-κB and NLRP3, followed by statistical analysis of the average optical density values of (B) RANKL and (C) OPG, and (D) the RANKL/OPG ratio (n=5). Scale bar, 200 μ m. (E) Immunofluorescence staining was used to detect the expression levels of CD68 and CD163 in macrophages cocultured with PDLSCs, iPDLSCs, and iPDLSCs after the downregulation of Fn14, NF-κB and NLRP3, followed by statistical analysis of the average optical density values of (F) CD68 and (G) CD163 (n=5). Scale bar, 200 μ m. Statistical analysis was performed using a one-way ANOVA. * P<0.05; ** P<0.01; **** P<0.0001. Data are presented as the mean ± SD. Fn14, fibroblast growth factor-inducible 14; iPDLSC, inflammatory PDLSC; MOD, mean optical density; NLRP3, NOD-like receptor thermal protein domain-associated protein 3; ns, not significant; OPG, osteoprotegerin; PDLSC, periodontal ligament stem cell; RANKL, receptor activator of nuclear factor-κB ligand; sh, short hairpin RNA.

Article Snippet: The primary antibodies used for immunofluorescence staining included the mouse anti-receptor activator of nuclear factor-κB ligand (RANKL) antibody (cat. no. sc-52950; Santa Cruz Biotechnology, Inc.), rabbit anti-osteoprotegerin (OPG) antibody (cat. no. ab73400; Abcam), mouse anti-CD68 antibody (cat. no. ab201340; Abcam) and rabbit anti-CD163 antibody (cat. no. ab182422; Abcam).

Techniques: Inhibition, Immunofluorescence, Staining, Expressing, shRNA